Affinity of Hydrogel Nanoparticles to Lysozyme
10.11895/j.issn.0253-3820.171368
- VernacularTitle:水凝胶纳米颗粒对溶菌酶的亲和研究
- Author:
Zhe WANG
1
;
Min XUE
;
Zi-Hui MENG
;
Tian-Tian JI
;
Teng-Sheng XIE
Author Information
1. 北京理工大学化学与化工学院
- Keywords:
Hydrogel nanoparticles;
Lysozyme;
Adsorption
- From:
Chinese Journal of Analytical Chemistry
2018;46(3):317-323
- CountryChina
- Language:Chinese
-
Abstract:
Hydrogel nanoparticles (NPs) were synthesized by precipitation polymerization method with N-isopropyl acrylamide (NIPAm), acrylic acid (Aac), N-tert butyl acrylamide (tBAM) and N,N'-methylene bisacrylamide(Bis) as thermosensitive monomer,negative monomer,hydrophobic monomer and crosslinker, respectively. The morphology of the resulting NPs was characterized by scanning electron microscopy(SEM),and the size and the particle size distribution were investigated by dynamic light scattering (DLS). The dynamics test was also carried out to investigate adsorption property of NPs. The results showed that NPs was spherical with uniform particle size and narrow distribution. NPs had the best adsorption performance to lysozyme when the monomer molar ratio was optimized to Aac 20%,tBAM 40%,NIPAm 38% and Bis 2%. Meanwhile, when the particle size of NPs decreased from 386. 20 nm to 77. 25 nm, the adsorption capacity increased gradually. The adsorption rate could reach up to 67.8% within 5 minutes. The thermosensitive of NPs provided a new candidate for the adsorption and separation of lysozyme with good reusability.
