Design and Evaluation of Online/offline Interface Based on Double Loop for Two-dimensional Chromatography
10.11895/j.issn.0253-3820.171182
- VernacularTitle:基于双回路技术的二维色谱在线/离线接口设计与评价
- Author:
Tao TANG
1
;
Wei-Bing ZHANG
;
Zheng ZHANG
;
Ming-Zhu XIA
;
Xue-Dong GONG
;
Feng-Yun WANG
;
Tong LI
Author Information
1. 南京理工大学工业化学研究所
- Keywords:
Double loop;
Online interface;
Offline interface;
Fraction collection;
Auto sampler;
Two-dimensional liquid chromatography
- From:
Chinese Journal of Analytical Chemistry
2018;46(3):311-316
- CountryChina
- Language:Chinese
-
Abstract:
By using a double loop technique based on a set of mechanical transmission components and a flow path system,a double function of injection and fraction collection was realized. On the basis of double loop technique,a novel type of online/offline interface for two-dimensional liquid chromatography was designed to construct an efficient separation system coupling two different separation modes with a higher peak capacity, and the functions of the interface were evaluated. By means of connecting the interface to an HPLC system, the multiple functions of injection, fraction collection and injection again were fulfilled for preparation and purity analysis of 4 kinds of aromatic compounds. As for combination of 2 sets of HPLC system,5 kinds of protein samples were separated preliminarily by strong cation exchange chromatography, and the components difficult to separate were collected and injected into reversed phase chromatographic system for further separation. Furthermore, the interface was applied to coupling two chromatographic systems in both strong cation exchange mode and microcolumn reversed-phase mode for the two-dimensional separation of bovine serum albumin enzymatic digest. When 1 mAU was set as the integral threshold,a total number of 292 peaks were identified. With the help of the online/offline interface, the preparation of microscale samples, fine separation of hardly separated samples and two-dimensional separation of complex samples were achieved flexibly. The result indicated that the system was a potent tool for the construction of two-dimensional chromatographic system and separation research.
