Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores
10.11895/j.issn.0253-3820.171299
- VernacularTitle:致孔剂溶解度参数对大孔层析介质的结构影响研究
- Author:
Meng-Fei LAN
1
;
Ning AN
;
Ying ZHAO
;
Wei CAO
;
Heng LI
;
Lan ZHAO
;
Yong-Dong HUANG
;
Rong-Yue ZHANG
Author Information
1. 北京石油化工学院化学工程学院/燃料清洁化及高效催化减排技术北京市重点实验室
- Keywords:
Porogen;
Solubility parameter;
Macroporous microspheres;
Protein;
Separation
- From:
Chinese Journal of Analytical Chemistry
2018;46(2):288-292
- CountryChina
- Language:Chinese
-
Abstract:
The macroporous microspheres were prepared through suspension polymerization and based on a copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate.The effect of porogen on the microspheres structure was evaluated in terms of pore size and surface area.Porogen contained dichloromethane (δ=9.7 (cal/cm3)1/2) and N-octanol (δ=10.3 (cal/cm3)1/2) which corresponded to a good and poor solvent,respectively.The solubility parameter of porogen was controlled in the range of 9.89-10.09 (cal/cm3)1/2.The pore size of microspheres increased with the difference value of solubility parameter between the polymer and the porogen.On the contrary,the surface area of microspheres decreased in this study.The anion exchange media was prepared through coupling poly(ethylene imine) in the microspheres,and the proteins transport was determined by frontal analysis method.The macroporous microspheres with 257 nm pore size could still afford a high proteins capacity (45.1 mg/mL).These macroporous supports showed a large potential in a rapid separation of proteins.
