Metabolomics Study on Susceptibility of Pneumonia in Children
10.11895/j.issn.0253-3820.171245
- VernacularTitle:基于代谢组学方法的小儿肺炎易感性研究
- Author:
Li-Li LIN
1
;
Shou-Chuan WANG
;
Yan YANG
;
Tong XIE
;
Xia ZHAO
;
Jing REN
;
Wei-Wei LI
;
Jia-Lei TAO
;
Si-Li FAN
;
Jin-Jun SHAN
Author Information
1. 江苏省儿童呼吸疾病(中医药)重点实验室
- Keywords:
Pneumonia inchildren;
Susceptibility;
Metabolomics;
Gas chromatography-mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2018;46(2):188-194,后插1-后插4
- CountryChina
- Language:Chinese
-
Abstract:
The changes of endogenous metabolites in urine samples that come from pneumonia patients of 6 months to 6 years old children were analyzed by metabolomics methods based on gas chromatography and mass spectrometry (GC-MS).The aim of this study was to analyze and study the pathogenesis of endogenous metabolites in children with pneumonia and the pathogenesis of pneumonia susceptibility.The urine samples were collected and divided into normal children group (NC group,n=29),first infection with pneumonia group (FIP group,n=35),and repeated infection with pneumonia group (RIP group,n=31).The urine metabolic profile of pneumonia was obtained by GC-MS.Principal component analysis(PCA) and partial least squares-discriminant analysis (PLS-DA) were used to analyze the data.The results were analyzed by one-way analysis of variance and Fold change.Finally,there was significant difference between the normal group and the pneumonia group,the significant metabolites were serine,histidine,proline,norleucine,glutamine,stearic acid,valine,isoleucine with p value<0.05 and Fold change>5,and indole-3-acetic acid,creatine,ethanolamine,mannosylglycerol and fructose were significant between the two pneumonia groups with p value<0.05.The urinary metabolites demonstrated that amino acid metabolism and glucose metabolism were the main metabolic pathways and responsible for the susceptibility to pneumonia.
