Development of Mass Spectrum Fingerprint Extraction Technology for Antibiotics in Surface Water
10.11895/j.issn.0253-3820.170302
- VernacularTitle:地表水中的抗生素质谱指纹提取方法的研究
- Author:
Si-Yi CHEN
1
;
Xin-Yan GUO
;
Wei ZHU
;
Na WANG
;
Ying WANG
;
De-Yang KONG
Author Information
1. 南京理工大学化工学院
- Keywords:
Solid phase extraction;
Ultra high performance liquid chromatography-tandem mass spectrometry;
Antibiotic;
Surface water
- From:
Chinese Journal of Analytical Chemistry
2018;46(2):178-187
- CountryChina
- Language:Chinese
-
Abstract:
An extract method for the fingerprint feature of 49 kinds of antibiotics belonging to multiple classes in surface water was developed.Water sample was purified and concentrated by tandem dual column (MAX and HLB),and qualitatively and quantitatively analyzed by ultra-high performance liquid chromatography-tandem mass spectrometric (SPE-UPLC-MS/MS) under multiple reaction monitoring (MRM) mode.The pretreatment was optimized in types of SPE column,loading pH,eluent and redissolution for multiclass antibiotics.The results showed that the linearity of target antibiotics was good in the range of 0.001-0.5 μg/mL (0.01-5 μg/mL for streptomycin).The recoveries were from 51.7% to 94.8%,and the relative standard deviations (RSDs) ranged from 2.19% to 9.67%.The limits of detection(LOD,S/N=3) were 0.01-3.23 μg/L and 0.05-3.43 μg/L and the limits of quantification (LOQ,S/N=10) were 0.04-10.8 μg/L and 0.17-11.4 μg/L in different redissolve solutions.This method was applied to the determination of antibiotics in water samples from 9 sites of Qinhuai River and Xuanwu Lake.
