Distinction of Cells Infected with Respiratory Syncytial Virus by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry
10.11895/j.issn.0253-3820.171011
- VernacularTitle:基质辅助激光解析/电离质谱用于呼吸道合胞病毒感染细胞的差异性分析
- Author:
Wen-Chan DENG
1
;
Guo-Bin HAN
;
Yuan-Fang LI
;
Cheng-Zhi HUANG
Author Information
1. 发光与实时分析化学教育部重点实验室
- Keywords:
Matrix-assisted laser desorption/ionization mass spectrometry;
Respiratory syncytial virus;
Biomarker
- From:
Chinese Journal of Analytical Chemistry
2018;46(2):165-169
- CountryChina
- Language:Chinese
-
Abstract:
Respiratory syncytial virus (RSV),which is a major cause of lower respiratory tract infections during infancy and childhood,is also an important pathogen for immunosuppression in elder and diseased areas.In order to research the pathogenesis of RSV,the matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS)was used to explore the difference between uninfected cells and virus-infected cells.The result showed that,in the molecular weight range of 5000~10000 Da,there are three component peaks expressed with significant difference in both normal group and infection group.One of the three components was up-regulated (m/z 6154.25) and the other two were down-regulated (m/z 7658.47 and 9259.82) after RSV infection.This result proved the obvious differences between the infected cells and untreated cells.The differential expression may provide a feasible method for RSV disease diagnosis and pathology research,and also provide scientific evidences for research on development of RSV therapeutic drugs.
