Effect of Osthole on cell proliferation and apoptosis in human nasopharyngeal carcinoma cell CNE2
10.16066/j.1672-7002.2018.02.004
- VernacularTitle:蛇床子素对人鼻咽癌细胞CNE2细胞增殖和凋亡的影响
- Author:
Jiongyu CHEN
1
;
Yiteng HUANG
;
Shanshan ZHUANG
;
Lin PENG
Author Information
1. 汕头大学医学院附属肿瘤医院中心实验室
- Keywords:
Nasopharyngeal Neoplasms;
Osthole;
Cell Proliferation;
Apoptosis
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2018;25(2):71-74
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the effect of Osthole on the proliferation and apoptosis in human nasopharyngeal carcinoma(NPC) cell, and to explore new treatment measures for nasopharyngeal carcinoma. METHODS Human NPC cell line CNE2 was treated with various concentrations of Osthole. MTT assay was used to investigate the cell viability, and apoptosis was detected by flow cytometry in CNE2 cells. Furthermore, the mRNA and protein expression levels of Bcl-2, Bax were determined by RT-PCR and western blot respectively. RESULTS Osthole induced significantly inhibitory effect on CNE2 cells at 24 h, 48 h and 72 h, and it was related with time and dose(P＜0.01). Following treatment of Osthole for 48 h, CNE2 cells showed significantly higher apoptosis rate than controls(P＜0.01). Meanwhile, both the mRNA and protein levels of Bax in Osthole-treated CNE2 cells were significantly higher than that of controls, while the level of Bcl-2 was downregulated, both of which changed with dose(P＜0.01). CONCLUSION The present study implied that Osthole can effectively inhibit proliferation and induce apoptosis in NPC cell lines CNE2.
