Clinical significance of detection for mutated BRAF gene in thyroid nodules based on amplification refractory mutation system
10.16066/j.1672-7002.2017.02.005
- VernacularTitle:基于ARMS法检测BRAF V600E突变在甲状腺乳头状癌中的临床价值
- Author:
Jinwang DING
1
;
Dingcun LUO
;
Yanping XUN
;
Wei WANG
;
Xiaocheng XU
;
Rongjing ZHOU
;
Yanping JIANG
;
Yiping XU
;
Wo ZHANG
;
Gang PAN
;
Liuqing YE
Author Information
1. 南京医科大学附属杭州医院杭州市第一人民医院肿瘤外科
- Keywords:
Thyroid Neoplasms;
Diagnosis;
Mutation;
amplification refractory mutation system;
BRAF gene
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2017;24(2):71-75
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the feasibility of detection for mutated BRAF V600E gene based on amplification refractory mutation system(ARMS),and to evaluate its clinical significance of BRAF V600E gene mutation in thyroid nodules.METHODS The method of ARMS was used to detect BRAF V600E mutation status in 179 patients with PTC and 115 patients with benign lesions.The diagnosis index of BRAF V600E mutation status for identifying the nature of the thyroid nodule was calculated.The potential correlation between BRAF V600E mutation and PTC clinicpathological characteristics was also analyzed.RESULTS Detection of BRAF V600E mutation status in thyroid lesions based on ARMS was feasible and believable.The positive rate of mutated BRAF V600E gene in PTC was 82.68％,whereas the rate in benign lesions was only 1.74％,indicating statistical differences between the two groups(x2=183.568,P＜0.01).The diagnostic sensitivity of BRAF V600E mutation was 82.68％,specificity was 98.26％,accuracy was 88.76％,and Youden index was 0.8094.There was no associations between the BRAF V600E mutation status and PTC clinicpathological characteristics(eg.gender,age,tumor size,numbers of lesions,bilateral lesions,extrathyroidal extension and lymph node metastasis).CONCLUSION Detection of BRAF V600E mutation based on ARMS has higher sensitivity and specificity in distinguishing PTC from benign lesions,indicating BRAF V600E gene is an ideal marker of PTC for clinical early diagnosis.
