Kinetic Parameters of Plasma Albumin Synthesis by Deuterated Water
10.16151/j.1007-810x.2018.01.014
- VernacularTitle:重水标记法测定大鼠血浆清蛋白合成动力的研究
- Author:
Ying TIAN
1
;
Chan-Fang MENG
;
Ming-Hui SHI
;
Qian-Qian DAI
;
Jing PENG
;
Yu CHEN
Author Information
1. 扬州大学旅游烹饪学院烹饪与营养系
- Keywords:
Deuterated water;
Albumin;
Fractional synthesis rate
- From:
Parenteral & Enteral Nutrition
2018;25(1):52-55,61
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To measure the kinetic parameters of murine plasma albumin synthesis by deuterated water (2H2O).Methods:Twenty SD rats received 2H2O-labelled intraperitoneal injection and drinking.At the end of the 1st,3rd,5th,6th,10th week,every 4 rats was respectively sacrificed by cardiac puncture for blood collection.Plasma albumin was purified by trichloroacetic acid and ethanol,identified by SDS-PAGE and hydrolyzed by hydrochloric acid.The alanine in albumin and plasma was separated,purified and derived.The deuterium enrichments of alanyl unit in albumin and alanine in plasma were determined by GC-MS.The fractional synthesis rate of albumin and synthetic dynamic equation were calculated.Results:The fractional synthesis rate of plasma albumin was 15.3％/w,and synthetic dynamic equation was ft =1.256 × (1-e-0.153t).Conclusion:The kinetic parameters of plasma albumin synthesis can be measured by 2H2O labeling method,which can be applied in the study of physiological and pathological metabolic mechanism.
