Changes of T cell immune mechanism in patients with acute exacerbation of chronic obstructive pulmonary disease
10.3969/j.issn.1671-8348.2018.17.011
- VernacularTitle:慢性阻塞性肺疾病急性加重期患者T细胞免疫机制变化初探
- Author:
Wen ZENG
1
;
Wen QIN
;
Dachun HU
;
Meihua LI
;
Shurong TANG
Author Information
1. 云南省昆明市第一人民医院检验科 650011
- Keywords:
pulmonary disease,chronic obstructive;
T-lymphocyte subsets,cytokines;
principal component analysis
- From:
Chongqing Medicine
2018;47(17):2298-2302
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of T lymphocyte immune balance in the patients with COPD.Methods Forty-eightpatients with AECOPD from January 2015 to December 2016 in this hospital were selected as study group and 30 healthy subjects as control.T cells and cytokines in peripheral blood were detected by flow cytometry and ELISA,respectively.The levels of them were compared between the two groups.The correlations of them and principal components were analyzed among the two groups.Results (1) CD8+T cells in the study group decreased [23.8％(17.5％,30.1％) vs.28.2％(23.8％,31.5％),P＜0.05],TregandTh17increased[8.5％(8.3％,9.0％) vs.5.6％(4.9％,6.1％),P＜0.01;2.9％(2.8％,3.0％) vs.1.2％(1.2％,1.4％),P＜0.01],respectively.(2)In the study group,IL-12 [54.97 pg/mL(31.20,161.59) pg/mL],IL-22 [17.70 pg/mL(15.60,35.99) pg/mL],IL-23[120.28 pg/mL(82.97,169.27) pg/mL],IL-23R [0.15 pg/mL(0.15,0.71)pg/mL]and TNF-α[1 000 pg/mL(1 000,1 000)pg/mL] were higher than that of the control group[31.20 pg/mL(31.20,56.23)pg/mL,15.60 pg/mL(15.60,15.60)pg/mL,78.10 pg/mL(78.10,99.08)pg/mL,0.15 pg/mL(0.15,0.15)pg/mL,722.16 pg/mL(494.25,941.44)pg/mL,P＜0.05].(3)Correlation analysis showed that there was a negative correlation between Th17 and Treg (r=-0.883,P＜0.01) in the study group (r=-0.883,P＜0.01),and IL-22 was positively correlated with IL-23 (r=0.340,P＜0.05).In the control group,there was a negative correlation between CD4+T cells and CD8+ T cells,Th17 and CD3+ T cells,Th17 and CD8+T cells (r was-0.578,-0.393 and-0.569,respectively,P＜0.05),and a positive correlation between Th17 and Treg,IL-23 and CD4+T cells,TNF-α and DNT(r=0.403,0.440 and 0.392,respectively.(4) There were 5 main components among each group,but the variable factors differed in the study group from the controls,as that:Th17/Treg,Th17,Treg vs.CD4+ T/CD8+ T cells,CD4+ T cells,CD8+ T cells and CD4+T/CD8+T cells,CD8+ T cells vs.Th17/Treg,Treg and CD4+ T cells,CD3+ T cells vs.IL-12,IL-23R andIL-12,IL-23R vs.CD3+ T cells and IL-23,IL-22 vs.TNF-α,DNT.Conclusion The increasing immune function of Th17 cells with IL-22 and IL-23 may be involved in the pathological mechanism of COPD,which accompanied with the weakened toxicity of CD8+T cells and regulation of DNT.
