Effect of ica operator on adherence and biofilm formation of Staphylococcus epidermidis at orthopedic implants
10.3969/j.issn.1671-8348.2018.17.006
- VernacularTitle:ica操纵子对表皮葡萄球菌在不同骨科植入物表面黏附和生物膜形成的影响
- Author:
Peng CHEN
1
;
Bo LI
;
Zhi PENG
;
Gan GAO
;
Sheng CHEN
Author Information
1. 贵州省人民医院骨科,贵阳550002
- Keywords:
staphylococcus epidermidis;
ica gene;
prostheses and implants;
cell adhesion;
biofilms
- From:
Chongqing Medicine
2018;47(17):2275-2278,2284
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ica operon on adherence and biofilm formation of Staphylococcus epidermidis(S.epidermidis)at orthopedic implants,and to provide theoretical basis for the use of clinical orthopedic materials.Methods The icaADBC genes of S.epidermidis were amplified by PCR.The expression of ica genes was detected by fluorescence quantitative PCR.The contents of polysaccharide intercellular adhesion (PIA) were determined by phenol-sulfuric acid method.The ability of bacterial adherence was detected by colony counting method and the ability of biofilm formation was measured by crystal violet staining method.Results The icaADBC genes were presented and expressed in eleven S.epidermidis strains.The strains with high icaABDC gene expression had higher PIA product.S.epidermidis isolates had the strongest adherence and biofilm formation ability on bone material,followed by titanium and stainless steel.The expression of icaA gene correlated with the biofilm formation of bacteria on three biomaterials.Conclusion S.epidermidis isolates had the different adherence and biofilm formation on different biomaterial implants.Ica gene regulates the biofilm formation of S.epidermidis by regulating the expression of PIA.
