miRNA-24-3P affect RAW264.7 cell function by modulating SP1

VernacularTitle:miRNA-24-3P靶向调节SP1对RAW264.7细胞功能的影响
Author: Dongliang CHENG ¹ ; Yuan LIANG ; Yanchen CHENG ; Di QING
Author Information

1. 郑州大学人民医院/河南省人民医院儿科
Keywords: miRNA-24-3P; respiratory distress syndrome,adult; SP1
From: Chongqing Medicine 2018;47(17):2257-2260,2265
CountryChina
Language:Chinese
Abstract: Objective To discuss the mechanism of miR-24-3P in the process of acute respiratory distress syndrome (ARDS).Methods miR-24-3P mimics,miR-24-3P inhibitor and their negative controls were transfected in RAW264.7 cells respectively:The relative expression of miR-24-3P in each cell was detected.The relative expression of TNF-α,IL-6,SP1 and NF-κB were detected in each cell,and the regulation of miR-24-3P on the target gene SP1 was detected by dual luciferase reporter gene.Results The expression level of TNF-α mRNA and IL-6 mRNA in each group was statistically significant (P＜0.05).The relative expression level of SP1 mRNA and NF-κB mRNA in each group was not statistically significant (P＞0.05).The expression of SP1 in each group was statistically significant (P＜0.05).The gene analysis of dual luciferase reporter showed that the fluorescence activity was significantly inhibited after cotransfection of miR-24-3P and SP1 in HEK293 cells(P＜0.05).Conclusion miR-24-3P can play an important role in the process of ARDS by influencing the translation of SP1 gene and affecting the release of inflammatory mediators.