The influence of M2 macrophages on tumor-promoting effect of gastric cancer-derived mesenchymal stem cells in gastric cancer microenvironment
10.3969/j.issn.1671-8348.2018.16.004
- VernacularTitle:胃癌微环境中M2亚型巨噬细胞对胃癌间充质干细胞促肿瘤作用的影响
- Author:
Zhaodong SUN
1
;
Ting ZHANG
;
Juan HUO
;
Wei LI
Author Information
1. 江苏省连云港市第一人民医院检验科 222001
- Keywords:
stomach neoplasms;
mesenchymal stem cells;
macrophages;
microenvironment
- From:
Chongqing Medicine
2018;47(16):2126-2130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of M2 macrophages resident in gastric cancer microenvironment on the tumor-promoting effect of human gastric cancer-derived mesenchymal stem cells (GC-MSCs).Methods Macrophages in BALB/c mice were depleted by using clodronate liposomes.The tumor volumes and weights in nude mice co-injected with GC-MSCs and BGC-823 with and without macrophage depletion were recorded.Tumor tissues of nude mice and gastric cancer patients were collected,and M2 macrophage-associated genes and proteins were detected by RT-PCR and western blot.Furthermore,the regulating effect of GC-MSCs on macrophage polarization to M2-subtype was validated in the co-culture experiment in vitro.Results Tumor growth in GC-MSCs co-injected mice was significantly inhibited by macrophage depletion (P=0.009).Results of RT-PCR and western blot showed that the transcription and expression of M2 macrophage-associated proteins were significantly higher in tumor tissues from GC-MSCs co-injected mice than those in the control group.Moreover,the transcription and expression levels of M2 macrophage-associated proteins were also high-er in gastric cancer tissues than those in the corresponding adjacent normal tissues.After co-culture with GC-MSCs directly,the expressions of M2 macrophage-associated proteins were significantly up-regulated in THP-1-derived macrophages.Conclusion M2 macrophages in gastric cancer microenvironment might play a critical role in the tumor-promoting effect of GC-MSCs.
