Analysis study of serum miRNA expression profile in patients with non-obstructive azoospermia
10.3969/j.issn.1671-8348.2018.15.010
- VernacularTitle:非阻塞性无精症患者血清miRNA表达谱分析研究
- Author:
Wenzhong ZHAO
1
;
Zhaofan LUO
;
Wenting FU
;
Ruishan WU
;
An ZHONG
;
Yu ZHOU
Author Information
1. 广东省计划生育科学技术研究所国家卫计委男性生殖与遗传重点实验室
- Keywords:
non-obstructive azoospermia;
serum;
microRNAs;
expression profile
- From:
Chongqing Medicine
2018;47(15):2011-2015
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the serum miRNA expression levels in non-obstructive azoospermia (NOA) patients and healthy sperm donors.Methods Serum miRNA levels in NOA patients and healthy sperm donors were analyzed by adopting the miRNA expression profiles chip.The data were processed and an alyzed by using the GenePix proV6.0 software to find out the differentially expressed miRNA,then the difference was verified by RQ-PCR,finally the bioinformatic software was utilized to predict the miRNA target gene.Results Compared to healthy sperm donors,71 cases of NOA had miRNAs expression difference,miRNA expression were increased in 47 cases and miRNAs expression was decreased in 24 cases.Moreover,Realtime PCR analysis verified the chip accuracy.The bioinformatic software target gene prediction showed that the potential target gene of these differential miRNA were involved in spermatogenesis.Conclusion The specific miRNA exists in serum miRNA of NOA,which helps to study the molecular mechanism of spermatogenesis.
