Comparative experiment study of two kinds of methods for establishing SD rat chloasma model
10.3969/j.issn.1671-8348.2018.15.005
- VernacularTitle:两种方法建立SD大鼠黄褐斑模型的比较实验研究
- Author:
Zhibo DENG
1
;
Shiyi HE
;
Qianli WANG
;
Yuntao LI
;
Qiaoli HU
;
Yonghong DU
Author Information
1. 重庆医科大学生物医学工程学院省部共建国家重点实验室培育基地-重庆市超声医学工程重点实验室/重庆市生物医学工程学重点实验室
- Keywords:
chloasma;
ultraviolet ray;
progesterone;
animal model;
rats,sprague-dawley
- From:
Chongqing Medicine
2018;47(15):1993-1996
- CountryChina
- Language:Chinese
-
Abstract:
Objective To comparatively analyze the SD rat chloasma model established by using the two methods of progesterone injection and ultraviolet radiation.Methods The rat chloasma model was established by adopting the high and low doses of progesterone injection and ultraviolet irradiation.The plaque area was observed and the skin section was observed by light microscope.The SOD and MDA levels closely related with chloasma melanin deposition were detected.Results The significant pigment deposition on the back skin in the high dose progesterone group was seen by naked eye and the scattered pigment deposition was seen in the low dose progesterone group;significant pigment deposition could be found in the ultraviolet group.Compared with the control group,the MDA level of various tissues in the high dose progesterone group was increased,while the SOD level was decreased,serum and skin SOD level in the low dose progesterone group was decreased,the difference was statistically significant(P＜0.05).Serum and skin SOD level in the high dose ultraviolet group was decreased,while the MDA level was increased,the skin SOD level in the low dose ultraviolet group was decreased(P＜0.05),but the difference between the two groups was not statistically significant (P＞0.05).The melanin grains were heaped up as the small black body under light microscope,which in the progesterone group showed the clutter distribution,the difference between the high dose group and low dose group was significant;which in the ultraviolet group showed the linear dense arrangement and the intergroup had no significant difference.Conclusion Progesterone injection and ultraviolet irradiation all can successfully establish the SD rat chloasma model,and the constructing model effect and stability of ultraviolet irradiation are better.
