The protective mechanisms of MEK1/2 inhibitor PD98059 on ox-LDL induced injury of HUVEC and the influence on the expression of LOX-1
10.3969/j.issn.1671-8348.2018.13.007
- VernacularTitle:MEK1/2抑制剂PD98059对ox-LDL诱导HUVEC损伤的保护机制及对LOX-1表达影响
- Author:
Shan SUN
1
;
Hongling HU
;
Xiaoyu DUAN
;
Qinqin WU
;
Yingxia HE
;
Xiaofen BU
;
Xiaoyan MING
;
Fengqin YAN
;
Hong ZHU
Author Information
1. 华中科技大学同济医学院附属武汉中心医院综合二科
- Keywords:
lipoproteins,LDL;
receptors,LDL;
PD98059;
MEK1/2;
RhoA/ROCK
- From:
Chongqing Medicine
2018;47(13):1727-1730
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective mechanism of MEK1/2 inhibitor PD98059 on ox-LDL induced injury of human umbilical vein endothelial cells (HUVEC),and its influence on the expression of LOX-1.Methods HUVEC damage models were established by using ox-LDL and were treated with PD98059 later,divided into the negative control group,the ox-LDL group,the positive control group and the PD98059+ox-LDL group.The effect of inhibition of MEK1/2 on ox-LDL induced HUVEC damage was measured.Results Compared with the negative control group,the levels in the ox-LDL group of LOX-1,pMEK1/2,RhoA,ROCK1,ROCK2,TNF-α and IL-6 were increased significantly,the proliferations of HUVEC and the productions of NO were decreased (P＜0.05).Compared with the ox-LDL group,the levels in the positive control group and the PD98059+ox-LDL group of pMEK1/2,RhoA,ROCK1,ROCK2,TNF-α and IL-6 were decreased,the proliferation of HUVEC and the production of NO were increased (P＜0.05).Conclusion PD98059 inhibit the MEK1/2 signaling pathway to suppress the ox-LDL induced damage of HUVEC by decreasing the expression of LOX-1.
