Study on the mechanism of miRNA-129-5p in epithelial-mesenchymal transition of biliary atresia mouse
10.3969/j.issn.1671-8348.2018.13.006
- VernacularTitle:miRNA-129-5p干扰技术对胆道闭锁小鼠胆道EMT作用机制的研究
- Author:
Jianyao WANG
1
;
Bin WANG
;
Zhouguang WU
;
Xiaoshuo YE
;
Zimin CHEN
;
Qi FENG
;
Dong LIU
;
Jun YAO
Author Information
1. 广东省深圳市儿童医院普外二科 518000
- Keywords:
biliary atresia;
miRNA-129-5 p;
epithelium-mesenchymal transition
- From:
Chongqing Medicine
2018;47(13):1723-1726
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of miRNA-129-5p in epithelial-mesenchymal transition (EMT)of biliary atresia.Methods Constructed bile duct epithelial EMT cell model (the experimental group) induced by TGF-β1,detected the expressions of EMT related markers and miRNA-129-5p.While miRNA-129-5p precursor was transfected,the expressions of EMT related markers and extracellular matrix were contrasted between the original and the renovated biliary epithelial cells.Results In the experimental group,extrahepatic bile duct showedEMT,the expression of miRNA-129-5p was decreased (P＜0.05),overexpression of miRNA-129-5p could inhibit the progression of EMT (P＜0.05).Conclusion miRNA-129-5p may relate to EMT by regulating the expression of TGF-β1.
