Ezrin promotes metastasis of lung cancer via L1CAM signaling pathway

VernacularTitle:埃兹蛋白通过L1细胞黏附分子信号通路促进肺癌转移
Author: Hui FENG ¹ ; Minggen JIN ; Tiefeng JIN
Author Information

1. 延边大学附属医院重症医学科
Keywords: lung neoplasms; Ezrin; L1CAM; metastasis
From: Chongqing Medicine 2018;47(12):1589-1591
CountryChina
Language:Chinese
Abstract: Objective To investigate the molecular mechanism of Ezrin in promoting human lung cancer metastasis.Methods The expression of Ezrin in lung cancer cell lines was detected by using RT-PCR and western blot;the cell scratch test was used to detect the effect of Ezrin on the migration ability of lung cancer cells;the mechanism of Ezrin for regulating L1CAM was detected by western blot.Results The western blot detection results showed that compared with the low metastatic lung cancer cell line H460 and EBC-1,the expression of Ezrin protein the high metastatic lung cancer cell line 95D and PC9 were higher (P＜0.05).After silencing the expression of Ezrin in 95D cells (siEzrin-95D) by using the genetic method,the cell scratch test showed that the migration ability of siEzrin-95D cells was significantly weakened compared with 95D cells (P＜0.05).Compared with 95D cells and H460 cells,after genetically silencing the Ezzin expression in 95D and H460 cells,the L1CAM expression was significantly down-regulated (P＜0.05).Conclusion Ezrin promotes lung cancer metastasis possibly by regulating the expression of L1CAM.