Expression of Nodal and its receptors in mouse tissues and organs at different ontogenetic stages
10.3969/j.issn.1671-8348.2018.12.003
- VernacularTitle:Nodal及其受体在不同发育期小鼠组织器官中的表达
- Author:
Ke WANG
1
;
Min ZHU
;
Hong GU
;
Nianfeng LI
;
Xianglin HU
;
Fancai ZENG
Author Information
1. 西南医科大学临床医学院
- Keywords:
Nodal signaling;
mice;
ontogenesis;
protein expression
- From:
Chongqing Medicine
2018;47(12):1575-1579
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Nodal and its receptors in different tissues and organs of different development stages of mice.Methods Ten pairs(male and female each for a pair) of mice were divided into the four groups:3 pairs served as adult mice group,the rest 7 pairs were allowed to mate,among them 3 pregnant mice served as the fetal group,and 3 pregnant mice served as the neonatal group and 1 pregnant mouse served as filial group.The multiple tissues and organs such as brain,liver,kidney,heart,lung were selected from fetal,neonatal,filial and adult mice for preparing the protein samples.Western blot was performed to detect the expression of Nodal and its type Ⅰ receptors of ALK7 and ALK4 as well as auxiliary receptor Cripto-1.Results Only cerebrum,cerebellum,liver and kidney had Nodal express in the four different mouse ontogenetic stages,in which only liver and kidney simultaneously expressed Nodal and its receptor protein in the whole four ontogenetic stages.Besides,most tissues and organs of adult mice expressed Nodal and its receptor protein,which was significantly different from the fetal,neonatal and filial mice.Conclusion Nodal signaling might have a certain effect on the growth and development of mouse liver and kidney during the late development stage.
