Feasibility of CM-Dil labeling bone-marrow mesenchymal stem cells in vitro
10.3969/j.issn.1671-8348.2018.11.002
- VernacularTitle:CM-Dil标记示踪间充质干细胞可行性的体外试验
- Author:
Xiaoying CHEN
1
;
Xinran LI
;
Qing WANG
;
Xiafei FU
Author Information
1. 南方医科大学珠江医院妇产科
- Keywords:
bone marrow mesenchymal stem cell;
CM-Dil;
cell label
- From:
Chongqing Medicine
2018;47(11):1436-1439
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate feasibility of CM-Dil labeling mesenchymal stem cells in vitro.Methods We separate,culture and identify rat bone marrow mesenchymal stem cells,after labeling MSCs with CM-Dil concentration respectively in 1,2,4μg/mL,observe label rate of MSCs after 15 min,24 h,48 h,72 h,5 passage and 8 passage,then detect cell cycle with FCM and cell proliferation capability with CCK8.Results MSC cultured with the whole bone marrow adherent method were strongly positive for CD44,CD29 and negative for CD34,CD45.The label rates of MSC 15 min after labeled with CM-Dil 1,2,4 μg/mL were respectively (31.60±1.25)％,(88.73±1.79)％,(96.89±1.31)％,and there has statistically difference (F=1 757.21,P=0.000);the growth curve of the 4 groups was similar,and the percentages of G1 phase,S phase and G2/M phase between the 4 groups have no statistically difference (P＞0.05).Conclusion CM-Dil in concentration 4 μg/mL has high label rate and low cytotoxicity,therefore could be a efficient and stable method of labeling MSCs.
