Effect of microRNA-134-5p targeting EGFR on growth of ovarian cancer SKOV3 and A2780 cells
10.3969/j.issn.1671-8348.2018.10.002
- VernacularTitle:微小RNA-134-5p靶向作用于EGFR对卵巢癌SKOV3和A2780细胞生长的影响
- Author:
Jiying TANG
1
;
Ping CHEN
;
Xiaojun CAI
;
Xuanbin WANG
;
Fengjun CAO
;
Li ZHANG
Author Information
1. 湖北医药学院附属人民医院肿瘤中心
- Keywords:
microRNAs;
ovarian neoplasms;
genes,tumor suppressor;
receptor,epidermal growth factor;
SKOV3;
A2780
- From:
Chongqing Medicine
2018;47(10):1301-1304
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of microRNA-134-5p (miR-134-5p) targeting epidermal growth factor receptor (EGFR) on the growth of ovarian cancer cells.Methods The ovarian cancer cell lines SKOV3 and A2780 served as the study objects and were divided into the control group (transfecting miR-NC) and experimental group (transfecting miR-134-5p) according to the treatment method.The expression levels of EGFR gene and downstream target protein were detected by qRT-PCR and western blot.The cell cycle distribution and apoptosis were detected by flow cytometry.The proliferation ability of ovarian cancer cells was detected by MTT assay and colony forming assay.Results The expressions of EGFR and downstream target protein in the experimental group were significantly down-regulated.EGFR mRNA in SKOV3 cells was downregulated to 48％ (P＜0.05),and EGFR mRNA in A2780 cells was down-regulated to 47％ (P＜0.05).The cell cycle of cells in the experimental group was significantly inhibited (P＜0.05),and miR-134-5p induced apoptosis through the EGFR target protein (P＜0.05).The proliferation activity and colony forming ability of the experimental group were significantly inhibited (P＜0.05).Conclusion miR-134-5p could promote the cellular cycle arrest and apoptosis,and reduces the proliferation ability of ovarian cancer cells by targetedly inhibiting the EGFR gene.
