Study on effect of quercetin on expression of typeⅠ,Ⅲ collagen in human hepatic stellate cells and cell cycle
10.3969/j.issn.1671-8348.2018.09.009
- VernacularTitle:槲皮素对人肝星状细胞Ⅰ、Ⅲ型胶原蛋白表达的影响与其细胞周期研究
- Author:
Wei JIANG
1
;
Zhilin DENG
;
Fuchang LI
Author Information
1. 四川省简阳市中医医院外科 641400
- Keywords:
liver;
hepatic stellate cell;
quercetin;
collagen;
cell cycle
- From:
Chongqing Medicine
2018;47(9):1179-1182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore effect of traditional Chinese medicine extract quercetin on the cell cycle and expression of typeⅠ,Ⅲ collagen in hepatic stellate cells,and to analyze the action mechanism of quercetin on liver fibrosis and cirrhosis.Methods The human hepatic stellate cells were cultured in vitro,divided into the control group(non-interference)and experimental group (quercetin interference).Then the cell growth characteristics were observed.The expression of collagenⅠ,Ⅲ was detected by using the immunohistochemistry and immunofluorescent techniques,and the cell cycle was examined by flow cytometry(FCM).Results (1)The cell nucleus was semi-quantitatively counted according to large,middle and small types.The results showed that the differ-ence between the experiment group and control group was statistically significant(P<0.05);(2)the immunohistochemistry and immunofluorescent results showed that type Ⅰcollagen expression by the absorbance,positive area ratio(PN-area)and fluorescent intensity had statistical difference between the experiment group and control group(P<0.05);while the typeⅢ collagen expression had no statistical difference between the experiment group and control group(P>0.05);(3)the cellular cycle detected by flow cy-tometry showed that the ratio of G1cells had statistical difference between the control group and experiment group(P<0.05).Con-clusion Quercetin has the regulating effect on the cell cycle and collagen expression in hepatic stellate cells.
