Reprogramming effects of two kinds of human odontogenic induced pluripotent stem cells and comparison of biological characteristics
10.3969/j.issn.1671-8348.2018.08.019
- VernacularTitle:两种人牙源性iPSCs的重编程效应及生物学特性比较
- Author:
Yu GUO
1
;
Jingshu XU
;
Qingyuan DAI
;
Xiaobing TAN
Author Information
1. 云南省第一人民医院口腔医学中心
- Keywords:
induced pluripotent stem cells;
dental pulp stem cells;
stem cells from apical papilla;
Sendai virus;
reprogram-ming
- From:
Chongqing Medicine
2018;47(8):1070-1073
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the reprogramming effects and biological characteristics of two types of human odontogen-ic induced pluripotent stem cells(iPSCs).Methods Human dental pulp stem cells(DPSCs)and stem cells from apical papilla (SCAP)were isolated and primarily cultured.The Sendai reprogramming system was utilized to induce DPSCs and SCAP into iP-SCs.The morphology,reprogramming efficiency,reprogramming and time were compared between human DPSCs-iPSCs and SCAP-iPSCs.The SeV and exogenous transcriptional gene expression were detected by RT-PCR.Results Human DPSCs and SCAP were reprogrammed as iPSCs with classical ES-like clonal morphology.The reprogramming efficiencies were(0.68 ± 0.02)% and(0.7 ± 0.01)% respectively,the difference was not statistically significant(P>0.05).The reprogramming time was(26.0 ± 2.1)d and (27.0 ± 1.4)d respectively,the difference was not statistically significant(P>0.05).The RT-PCR results showed that no expres-sion of exogenous virus or transcriptional gene sequence in both iPSCs.Conclusion Human DPSCs and SCAP can be reprogrammed as virus-free and transgene-free iPSCs,which are the ideal sources of iPSCs.
