Study on expression of ASC and Caspase-1 in peripheral blood of patients with primary biliary cirrhosis
10.3969/j.issn.1671-8348.2018.08.011
- VernacularTitle:ASC与Caspase-1在PBC患者外周血中的表达研究
- Author:
Jia FAN
1
;
Erchuan ZHAO
;
Zhenxuan YE
;
Hongmei LI
;
Zhijing REN
;
Hua ZHANG
Author Information
1. 贵州医科大学临检教研室
- Keywords:
liver cirrhosis;
biliary;
cysteine endopeptidases;
ASC;
pyrophosphate sequencing
- From:
Chongqing Medicine
2018;47(8):1044-1048
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of apoptosis-associated speck-like protein containing a caspase recruitment do-main(ASC)and Caspase-1 on the pathogenesis of primary biliary cirrhosis(PBC).Methods The real-time PCR,Western blot,py-rophosphate sequencing and ELISA were adopted to respectively detect the relative expressions of mRNA and protein of peripheral blood mononuclear cells(PBMS)Caspase-1 and ASC as well as the methylation status of ASC promoter region and plasma Caspase-1 and IL-18 expression levels in 30 cases of PBC and healthy controls.Results The mRNA and protein expressions of PBMC Caspase-1 and ASC in the PBC group were significantly higher than those in the control group(P<0.05).The methylation rate of ASC promoter Island1(ISI)was significantly lower than that of the control group(P<0.05),which of Island 2(IS2)was smaller than the background value and had no methylation occurrence.The levels of plasma Caspase-1 and IL-18 in the PBC group were sig-nificantly higher than those in the control group(P<0.05).The ASC mRNA in the PBC group was significantly correlated with the Caspase-1 mRNA expression(P<0.05);the methylation rates at loci 1,2,4,5 of ASC promoter region CpG island were nega-tively correlated with ASC mRNA expression(P< 0.05),and which at loci 3,6 had no correlation with their expressions(P>0.05);plasma Caspase-1 and IL-18 levels showed the obviously positive correlation.Conclusion ASC and Caspase-1 are involved in the immune inflammatory response in PBC.
