Effect of C3G overexpression on proliferation and apoptosis of high glucose-induced H9C2 cardiomyocytes
10.3969/j.issn.1671-8348.2018.08.008
- VernacularTitle:过表达C3G对高糖诱导H9C2心肌细胞增殖和凋亡的影响
- Author:
Xu ZHANG
1
;
Wangsheng CHEN
;
Mengjiao ZHANG
;
Jiao NIE
;
Xiu CHEN
Author Information
1. 西南医科大学附属医院老年病科
- Keywords:
H9C2 cardiomyocyte;
guanine nucleotide-releasing factor 2;
high glucose;
cell survivability
- From:
Chongqing Medicine
2018;47(8):1033-1035
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Crk-SH3 domain guanine exchange factor(C3G)overexpression on the pro-liferation and apoptosis of high glucose-induced H9C2 cardiomyocytes.Methods H9C2 cardiomyocytes were transiently transfected with pCXN2-Flag(empty plasmid)and pCXN2-Flag-hC3G(human C3G mRNA)plasmids,then conducted high glucose(HG)in-tervention.The experiment was divided into the blank group,empty vector group,C3G overexpression group,blank + HG group, empty vector + HG group and C3G overexpression+ HG group.The C3G protein expressions,apoptosis and proliferation rate were respectively detected in each H9C2 cardiomyocytes groups.Results The proliferation rate in the blank+ HG group and empty vec-tor+ HG group were significantly decreased compared with the blank group and empty vector group,while the apoptosis rate was significantly increased.Compared with blank group and empty vector group,blank + HG and empty vector + HG group,the C3G protein expression and proliferation rate in the C3G overexpression group and C3G overexpression+ HG group were increased sig-nificantly,while the apoptosis rate was decreased significantly.Conclusion High glucose inhibits H9C2 myocardial cell proliferation and promots its apoptosis;furthermore,C3G overexpression can reversed the decrease of high glucose-induced H9C2 cardiomyocyte prolifer-ation rate and apoptosis increase.C3G overexpression can promote the survivability of high glucose-induced H9C2 cardiomyocytes.
