Study on insulin for regulating VGLUT2 gene expression in rat pancreatic β cell
10.3969/j.issn.1671-8348.2018.08.004
- VernacularTitle:胰岛素对大鼠胰岛β细胞VGLUT2基因表达的调控研究
- Author:
Shuwen WANG
1
;
Tao LI
;
Hongcheng LIN
;
Feng YANG
;
Rongwen LIN
;
Yuanshou CHEN
Author Information
1. 广东医科大学第二临床医学院
- Keywords:
diabetes mellitus;
insulin;
vesicular glutamate transport protein 2;
receptor;
insulin;
insulin receptor substrate
- From:
Chongqing Medicine
2018;47(8):1019-1022
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulation of insulin VGLUT2 gene expression in pancreatic β cells and its exactly mechanism.Methods The rat pancreatic β cell line RIN-5F was treated by different insulin concentrations and different siRNA.The changes of VGLUT2 mRNA and protein expression levels were detected by adopting real-time qPCR and Western blot.Results In-sulin with a concentration of 100,200 nmol/L significantly inhibited the expressions of VGLUT2 mRNA and protein in RIN-5RF cells(P<0.05),moreover the inhibiting effect was most significant at 100 nmol/L.After 100 nmol/L insulin treatment,the expres-sions of VGLUT2 mRNA and protein at 12,18,24 h were significantly inhibited compared with that at 0 h(P<0.05).Compared with the Blank group,Lip2000 group and Control-siRNA group,after interfering RIN-5F by using IR-siRNA,IRS1-siRNA and IRS2-siRNA,the inhibition situation of VGLUT2 mRNA and protein expressions by 100 nmol/L insulin in each group was signifi-cantly recovered(P<0.05).Conclusion Insulin at low concentration could inhibit VGLUT 2 gene expression in rat pancreatic β cell line RIN-5F.
