Effect of Xiaoqinglong Decoction on the expression of TSLP in airway epithelial cells of asthmatic model mice
10.3969/j.issn.1671-8348.2018.08.003
- VernacularTitle:小青龙汤对哮喘模型小鼠气道上皮细胞表达TSLP的影响研究
- Author:
Guihua SONG
1
;
Yan ZHANG
;
Suping YU
;
Weigang LV
;
Zhiwei GUAN
;
Xiaofeng MEI
;
Mengmeng SUN
;
Jing WANG
Author Information
1. 河南中医药大学第一附属医院儿科
- Keywords:
asthma;
thymic stromal lymphopoietin;
Xiaoqinglong Decoction
- From:
Chongqing Medicine
2018;47(8):1016-1018,1022
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Xiaoqinglong Decoction on the expression of TSLP in airway epithelial cells of asthmatic model mice.Methods Thirty healthy female BALB/C mice were divided into the blank control group,asthma model group and Xiaoqinglong Decoction group,10 cases in each group.The each group adopted corresponding intervention measures. Then the bronchial and lung tissues were taken.The expression level of bronchial TSLP was detected by immunofluorescence,and the expression level of TSLP mRNA and protein in lung tissue was detected by qRT-PCR and Western Blot.Results The mouse bronchial epithelial cell membrane TSLP staining was strongly positive in the asthma model group,which was weakly positive in the Xiaoqinglong Decoction group and negative in the blank control group.Compared with the asthma model group,mice lung tissue TSLP mRNA expression level in the Xiaoqinglong Decoction group and blank control group was decreased(P<0.05),the Xiaoqin-glong Decoction group was slightly higher than the blank control group,but the inter-group difference was not statistically signifi-cant(P>0.05).The expression level of lung tissue TSLP protein in the Xiaoqinglong Decoction group and blank control group was lower than that in the asthma model group(P<0.05),the Xiaoqinglong Decoction group was slightly higher than the blank control group,but the inter-group difference was not statistically significant(P>0.05).Conclusion Xiaoqinglong decoction has obvious inhibiting effect on the expression of TSLP in airway epithelial cells of asthmatic model mice.
