Construction and identification of UAS-Hey transgenic fly strains

VernacularTitle:UAS-Hey转基因果蝇品系的构建与鉴定
Author: Songnian LIU ¹ ; Linghua JING ; Xing WU ; Xin ZHAO
Author Information

1. 河南科技大学临床医学院/河南科技大学第一附属医院急诊科
Keywords: Hey gene; transgenic fly; microinjection; cardiovascular development
From: Chongqing Medicine 2018;47(5):654-656,659
CountryChina
Language:Chinese
Abstract: Objective To construct the UAS-Hey transgenic fly strains to provide a tool for researching the function of Hey gene in the fly development.Methods The Hey gene coding sequence was amplified by reverse transcription PCR and subcloned into pUAST expression vector.The pUAS-Hey recombinant plasmid was constructed and microinjected into the embryos of wild type flies.The UAS-Hey transgenic flies with red eyes were screened out with mini-white marker,then the balancing and mapping of these transgenic strains were performed.The identification was performed by using the PCR amplification method.Results The pUAS-Hey recombinant plasmid was successfully constructed,and seven independent transgenic strains were obtained by microinjection and transgenic fly screening and balance.PCR analysis confirmed that the P[mini-white,UAS-Hey] was integrated into genomes of transgenic strains and was in expressible region.Conclusion The UAS-Hey transgenic flies are successfully constructed,which lays the foundation for further studies of the function and regulation of Hey gene with GAL4/UAS system.