miRNA-125b over-expression for promoting proliferation and invasion ability of lung cancer A549 cells and its mechanism
10.3969/j.issn.1671-8348.2018.05.009
- VernacularTitle:过表达miRNA-125b促进肺癌细胞A549的增殖、侵袭能力及其机制研究
- Author:
Huayong ZHANG
1
;
Qiang HAN
;
Qiaomin LI
;
Beilong ZHONG
Author Information
1. 中山大学附属第五医院胸心外科
- Keywords:
micorRNA-125b;
lung neoplasms;
cell proliferation;
invasion ability
- From:
Chongqing Medicine
2018;47(5):604-606
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of miRNA-125b over-expression on proliferation and invasion ability of lung cancer A549 cells and its mechanism.Methods A549 cells were divided into 3 groups:miRNA-125b group(transfected with miRNA-125b mimics),NC group(transfected with NC mimics) and blank group(same volume of GIBCO serum mixed with transfection agent).The transfection and expression efficiency of miRNA-125b was detected with Q-PCR,the proliferation ability was detected with MTT,and the invasion ability was detected with the transwell chamber test.The expression level of BMF in A549 cells was detected with Western blot.Results Compared with the blank group,the expression level of miRNA-125b,proliferation ability and invasion ability in the miRNA-125b group were increased(P＜0.05);while the above indexes in the NC group demonstrated no significant change(P＞0.05).Compared with the blank group,the expression level of BMF in the miRNA-125b group was decreased (P＜0.05);while which in the NC group had no significant change(P＞0.05).Conclusion miRNA-125b can promote the proliferation and invasion ability of A549 cells via inhibiting the expression of BMF.
