Effect of MIF antibody on aberrant crypt foci and colorectal carcinoma in rat
10.3969/j.issn.1671-8348.2018.05.007
- VernacularTitle:巨噬细胞移动抑制因子抗体对大鼠大肠异常隐窝病灶及大肠癌的影响
- Author:
Yuanyuan SHEN
1
;
Chengxia LIU
;
Aili WANG
;
Kai CHENG
;
Ranran ZHANG
Author Information
1. 滨州医学院附属医院消化内科
- Keywords:
macrophage migration inhibitory factor antibody;
aberrant crypt foci;
colorectal neoplasms
- From:
Chongqing Medicine
2018;47(5):598-600
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of macrophage migration inhibitory factor(MIF) antibody on the rat colonic aberrant crypt foci(ACF) induced by 1,2-dimethylhydrazine(DMH),carcinoma number and expression of MIF in rat colonic carcinogenesis.Methods Rat colonic carcinogenesis model was induced by DMH.In this model,the inhibitory effect of MIF antibody on the number of ACF and carcinoma was observed.ELISA and immunohistochemical staining were adopted to investigate the effect of MIF antibody in early cancerative intestinal mucosa and MIF expression after cancer formation.Results The number of ACF and carcinoma was significantly inhibited by MIF antibody intervention(P＜ 0.01).The expression of MIF in the colonic carcinoma model was significantly higher than that in the pre-carcinoma ACF model(P＜0.01).Applying MIF antibody could significantly inhibit the expression of MIF in both rat colonic ACF and colonic carcinoma model.Conclusion MIF antibody can significantly inhibit the rat colonic mucosal carcinogenesis,which may be related with inhibiting number of ACF and expression of MIF.MIF antibody may be expected to become a new target spot of precaution and treatment of colonic carcinoma.
