Influence of cyclophosphamide and its metabolites on PTEN gene after acting on ovarian cancer cell line SKOV3
10.3969/j.issn.1671-8348.2018.04.006
- VernacularTitle:环磷酰胺及其代谢产物作用于卵巢癌细胞SKOV3后对PTEN基因的影响
- Author:
Zhengfang XIONG
1
;
Bing LI
;
Xianghui ZENG
;
Liyun WANG
Author Information
1. 青海省人民医院生殖中心
- Keywords:
chromosome 10 loss of phosphatase and tension protein homologous genes;
cyclophosphamide;
acrolein;
phosphorylation;
activity
- From:
Chongqing Medicine
2018;47(4):453-455
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of cyclophosphamide(CP) and its metabolites acrolein(ACR) on PTEN gene deleted on chromosome 10 after acting on ovarian cancer cellsSKOV3.Methods Different concentrations of CP and ACR were selected to act on recombinant PTEN protein.The phosphorylation activity of PTEN was detected by PNPP.The expression of PTEN protein was detected by Western blot.The binding mode of drug with protein was detected by the biotin combined with protein;meanwhile the expression change of P53/TP53 in PTEN gene pathway was analyzed.The target protein was obtained by immunoprecipitation(IP) after different drug concentrations acting on the cells.The phosphorylation activity of the target protein was detected by high performance liquid chromatography(HPLC).Results After the drug metabolites acting on recombinant PTEN protein,the phosphorylation activity was decreased with the increase of drug concentration,while the expression of ACR antibody action was increased with the drug concentration elevation.The expression of protein and biotin in different experimental groups was increased with the increase of drug concentration.The PTEN phosphorylation activity was decreased with the drug concentration increased in cells,and so did the expression of TP53 protein.Conclusion CP metabolite ACR induces the cytotoxicity by inhibiting PTEN protein phosphorylation activity.
