Research on effect of GTPBP4 on biological behavior of hepatocellular carcinoma and its mechanism
10.3969/j.issn.1671-8348.2018.04.003
- VernacularTitle:GTPBP4对肝细胞癌生物学行为影响及其机制的研究
- Author:
Nuobei ZHANG
1
;
Xin CHEN
Author Information
1. 南昌大学第二附属医院消化内科
- Keywords:
GTPBP4;
carcinoma,hepatocellular;
biological behavior;
cell cycle
- From:
Chongqing Medicine
2018;47(4):439-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of GTPBP4 gene expression down-regulation in hepatocellular carcinoma (HCC) cell line SMMC-7721 on the intracellular and extracellular biological behaviors and its action mechanism.Methods The differences of GTPBP4 expression level in HCC tissues and paracancerous tissues were compared by using the immunohistochemical method.The expression of GTPBP4 mRNA in 4 kinds of HCC cell line(SMMC-7721,HEPG2,HUH-7,HEP3B) was detected by using the real-time fluorescent quantitative PCR(RT-PCR).The expression of GTPBP4 in HCC cell line SMMC-7721 was downregulated by using the RNA interference technique and the cellular biological behavior change was observed.Results In HCC histological chip,the expression level of GTPBP4 protein in HCC tissue was significantly higher than that in para-cancerous tissue.GT-PBP4 was also significantly up-regulated in 4 kinds of HCC cell line.After the expression of GTPBP4 was down-regulated,the proliferation ability of HCC cells was weakened and apoptosis was increased.The cells in S phase and G1.phase had no significant changes,but which in G2 phase were increased,the ability of in vitro clone formation was weakened,the nude mouse in vivo tumor formation capacity was weakened.The expressional profiles microarray results showed that 333 genes were changed in SMMC-7721 cells after GTPBP4 knockdown,in which the up-regulated genes were 134,the down-regulated genes were 199.The channel enrichment analysis found 10 signal transduction pathways of the enriched differential genes.Western blot showed that the expression levels of CCND1,CCND2,CDK6 and MDM2 were changed significantly after GTPBP4 expression down-regulation.Conclusion GT-PBP4 as a promoting HCC gene may influence HCC biological behavior possibly by regulating the expression of key gene in cell cycle.
