Analysis of Coxsackie virus A6 and A10 detection in hand foot and mouth disease in Guiyang area during 2015
10.3969/j.issn.1671-8348.2018.03.027
- VernacularTitle:贵阳地区2015年手足口病柯萨奇病毒A6、A10型的检测分析
- Author:
Xinglin YANG
1
;
Zhangping HONG
;
Yi WANG
;
Li LI
;
Yuedong LIANG
;
Hai HUANG
Author Information
1. 贵州省贵阳市公共卫生救治中心 550004
- Keywords:
hand,foot and mouth disease;
enterovirus A,human;
genotype
- From:
Chongqing Medicine
2018;47(3):374-376,380
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the enterovirus(EV) pathogenic composition of Coxsackie virus(CV)A6 and A10 causing hand foot and mouth disesse(HFMD) in Guiyang area during 2015 to provide a basis for the prevention and treatment of HFMD in local area.Methods The specific primers were respectively designed according to CVA6 and CVA10 sequence published in GenBank.The fluorescence quantitative RT-PCR method system was established.The gene sequencing method was used for conducting verification.Then this method was used to detect the clinical samples from 607 cases of HFMD.Results A total of 607 samples of suspected HFMD were detected,the overall positive rate was 59.47 ％ (361/607),in which EV71 accounted for 7.25 ％ (44/607),CVA16 for 11.37％(69/607),EV71+CVA16 double positive accounted for 0.16％ (1/607) and other EV for 40.69％ (247/607).The positive samples of CVA6,CVA10 and CVA6+CVA10 detected by the established real time fluorescence RT-PCR were 11 cases,71 cases and 1 case.Conclusion CVA6 and CVA10 are the main pathogens causing new onset HFMD in Guiyang area and the CVA10 monitoring should be strengthened.
