Effects of long term low-dose manganese exposure on mitochondria morphology and apoptosis of spermatogenic cells in testis of offspring rats
10.3969/j.issn.1671-8348.2018.03.015
- VernacularTitle:长期低剂量锰染毒对子代大鼠睾丸生精细胞线粒体状态和细胞凋亡的影响
- Author:
Qianxing WANG
1
;
Hui CHU
;
Mingming YU
;
Xianping ZHANG
Author Information
1. 遵义医学院细胞生物学教研室
- Keywords:
manganese;
seminiferous epithelium;
mitochondria;
apoptosis
- From:
Chongqing Medicine
2018;47(3):333-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of long term low-dose manganese exposure on testicular spermatogenic cell mitochondria morphology and apoptosis of male offspring rat.Methods Thirty-two healthy female SD rats were divided into the control group,low,middle and high dose groups.2,4 and 8 mg/kg MnCl2 · 4H2 O or normal saline was intraperitoneally injected for 8 weeks(once daily,5 d/week).The manganese exposure continued during the pregnant period and lactation period.Eight 12-week-old offspring male rats were killed in each group,the structure of seminiferous tubules and mitochondria morphology in spermatogenic cells were observed.The expression of Opa1,Drp1 and Caspase9,and the apoptosis of spermatogenic cells were detected.Results With the increase of administered-MnCl2 dosage,the number of spermatogenic cell layers decreased,spermatogenic cells arranged in disorder,the number reduced,etc;the mitochondria separation and swelling of spermatogenic cells were found in the middle and high dose groups;the expression of Opa1 was gradually decreased in the middle and high dose groups(P＜0.05,P＜0.01),while the expression of Drp1 and Caspase9 was gradually increased with the manganese dose increase(P＜0.05,P＜0.01);the apoptotic index of spermatogenic cells in the manganese exposure group was significantly increased with the increase of administeredMnCl2 dosage(P＜0.05).Conclusion Long term low dose of manganese exposure could regulate the expression of Opa1/Drp1 gene and affect the function of mitochondria,leads to apoptosis of spermatogenic cells in male offspring rat.
