Antiproliferative and apoptosis effect of atorvastatin on K562 cells
10.3969/j.issn.1671-8348.2018.03.003
- VernacularTitle:阿托伐他汀对K562细胞的抗增殖作用及凋亡作用的研究
- Author:
Chunfang KONG
1
;
Jianglong ZHOU
;
Weirong DING
;
Jianghua DING
;
Guoan CHEN
;
Hongbo CHENG
;
Chenghao JIN
Author Information
1. 江西省人民医院
- Keywords:
cell proliferation;
apoptosis;
atorvastatin;
K562 cells
- From:
Chongqing Medicine
2018;47(3):299-301,305
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of atorvastatin on the proliferation and apoptosis of K562 cells andto investigate its mechanisms.Methods The cells were treated by different concentrations of atorvastatin.The CCK-8 assay was employed to detect the cell proliferation.The cell apoptosis was detected by AnnexinV-FITC/PI dual staining;the flow cytometry was used to detect the cellular cycle;the activities of caspase-3,-8,-9 were detected by the colorimetric method;qRT-PCR was employed to measure the mRNA expression levels of Bcl-2 and PDCD5 in K562 cells.Results Atorvastatin could inhibit the proliferation of K562 cells in a time-and dose-dependent manner(P＜0.05);and induced the apoptosis of K562 cells,the percentage of G0/G1 phase cells was increased after atorvastatin treating k562 cells(P＜0.01),while the percentage of S phase cells was decreased(P＜0.01),moreover which showing the concentration dependence(P＜0.01);atorvastatin activated the caspase-3,-8,-9 (P＜0.01);down-regulated Bcl-2 mRNA expression and up-regulated PDCD5 mRNA expressionin a concentration dependence(P＜0.01).Conclusion Atorvastatin can inhibit the proliferation and induce apoptosis in K562 cells.
