Effects of tubacin on membrane surface structure of BMSCs and mechanical property
10.3969/j.issn.1671-8348.2018.02.004
- VernacularTitle:Tubacin对大鼠骨髓间充质干细胞膜表面结构及力学性能的影响
- Author:
Fang LU
1
;
Yanfang ZHOU
;
Xinsheng PENG
;
Chenchen ZHANG
;
Jiaqiang LIANG
Author Information
1. 广东医科大学病理生理学教研室
- Keywords:
mesenchymal stem cells;
microscopy,atomic force;
mechanics;
ultramicrostructure
- From:
Chongqing Medicine
2018;47(2):156-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect different concentrations of HDAC6 inhibitor tubacin on the proliferation,morphology and membrane surface ultrastructure of bone marrow mesenchymal stem cells(BMSCs).Methods Primary BMSCs were cultured.The P4 generation cells were taken for conducting the experiment.The different concentrations of tubacin were used to treat the cells fro 24 h.The cells survival rate was detected by MTT assay.The atomic force microscopy(AFM) was applied to observe the cellular morphology and surface ultramicrostructure and detect the mechanical property in different groups.Results The MTT results showed that low concentration of tubacin had the effect for promoting BMSC proliferation;the AFM results showed that compared with the control group,the height and width of BMSCs after treating by low concentration of tubacin,the membrane surface roughness was decreased and cellular hardness was increased.Conclusion Low concentration of tubacin can promote the BMSC proliferation,causes the changes of morphology and membrane surface ultramicrostructure,enhances the mechanical property and increases the cell implantation treatment efficiency.
