The impact of HSF2 on the development of lung cancer by promoting IL-10 expression
10.3969/j.issn.1671-8348.2018.01.015
- VernacularTitle:热休克转录因子2通过促进白细胞介素-10的表达对肺癌发生的影响
- Author:
Minjuan FAN
1
;
Yunhua ZHONG
;
Wen SHEN
;
Kaifeng YUAN
;
Guohou ZHAO
;
Shukun WANG
;
Linqiao WEN
Author Information
1. 昆明医科大学第二附属医院呼吸科
- Keywords:
lung neoplasms;
heat shock factor 2;
interleukin-10;
A549 cell
- From:
Chongqing Medicine
2018;47(1):43-45,48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact of heat shock factor 2 (HSF2) on the development of lung cancer by promoting interleukin (IL)-10 expression.Methods 50 lung cancer tissues and adjacent normal tissues selected from 50 patients,the expression level of mRNA and protein of HSF2 and IL-10 were respectively detected by RT-PCR,Western blot and Immunohistochemistry;To interfere with expression of HSF2 in A549 cells by siRNA,the expression level of IL-10 was detected by Western blot.Results Compared with the adjacent normal tissues,HSF2 of 76％ (38 of 50) cases were up-regulated (P＜0.01),IL-10 of 80％ (40 of 50) eases were up-regulated (P＜0.01),protein level consistent with mRNA level.The up-regulation expression of IL-10 in lung cancer tissues and HSF2 positively correlated (R2 =0.921 6).The expression of IL-10 in A549 cells was weakened through interference with HSF2 by siRNA.Conclusion HSF2 may contribute to the development of lung cancer by facilitating the expression of IL-10.
