In vitro study on effects of diosgenin from Dioscorea nipponica on mouse T lymphocytes
10.3969/j.issn.1671-8348.2018.01.007
- VernacularTitle:穿龙薯蓣皂苷元对小鼠T淋巴细胞影响的体外研究
- Author:
Ye WANG
1
;
Congmin FU
;
Lirong JIA
;
Songran LI
;
Xiaowei DANG
;
Xiufen MA
;
Bowen QIN
Author Information
1. 承德护理职业学院
- Keywords:
diosgenin;
T lymphocyte;
interleukin-2;
interferon type Ⅱ;
mRNA
- From:
Chongqing Medicine
2018;47(1):20-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of diosgenin(Dio) from Dioscorea nipponica on the proliferation in mouse spleen T lymphocytes and expressions of IL-2 and IFN-γmRNA,in order to investigate the immunity regulatory mechanism of Dio.Methods T lymphocytes stimulated by different concentrations of Dio and concanavalin A(ConA) were co-cultured.CCK-8 was used to detect the Dio effects on T lymphocyte proliferation.The RT-PCR method was adopted to detect the effect of Dio on expression of IL-2 and IFN-γmRNA.Results The Dio concentration in the range of 0.937 5-15.000 0 μg/mL had the inhibiting effect on T lymphocyte proliferation,Dio concentration in the range of 3.750 0-15.000 0μg/mL had the inhibiting effect on IFN-γ and IL-2 expression in T lymphocytes.With the Dio concentration increase,the inhibition effect was enhanced(P＜0.05).7.500 0 μg/mL was the best inhibition concentration.The inhibition effect was decreased when the concentration exceeding 7.500 0 μg/mL.Conclusion Dio has the inhibiting effect on T lymphocyte proliferation and expressions of IL-2 and IFN-γ mRNA.
