Study on mechanism of Dahuanglingxian Capsule for intervening gallstone formation by regulating ABCB11 and ABCC2
10.3969/j.issn.1671-8348.2018.01.002
- VernacularTitle:大黄灵仙胶囊调控ABCB11和ABCC2干预胆结石形成的作用机制研究
- Author:
Qianli TANG
1
;
Zhen LV
;
Bing WANG
;
Yu WANG
;
Shu WANG
;
Qingfeng SHU
;
Bin GE
;
Sizhen XIE
Author Information
1. 右江民族医学院/桂西高发病防治重点实验室
- Keywords:
gallstone;
Dahuanglinxian Capsules;
ABCB11;
ABCC2
- From:
Chongqing Medicine
2018;47(1):4-6,10
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Dahuanglinxian Capsule for intervening gallstone formation by regulating the expression levels of ABCB11 and ABCC2 mRNA and protein.Methods Forty male C57BL/6 mice were divided into the normal group(group N),model group (group M),ursodeoxycholic acid group (group U) and Dahuanglinxian Capsule treatment group (group D),10 cases in each group.The group N was fed with normal diet,while the group M,U and D were fed with lithogenic fodder for 8 weeks.Meanwhile the group U and D were given the medication intervention,once daily,for continuous 8 weeks of gavage.After successful modeling,mRNA and protein expression levels of ABCB11 and ABCC2 were detected by RT-PCR and immunohistochemistry.Results Compared with the other three groups,the expressions of ABCB11 and ABCC2 mRNA gene and protein in M group were significantly reduced(P＜0.01);while,there was no statistical difference in the expressions of ABCB11 and ABCC2 mRNA and protein between the group D and N(P＞0.05).Conclusion Dahuanglinxian Capsule can prevent the gallstone formation by regulating the expression of ABCB11 and ABCC2 mRNA and protein.
