Study on correlation between TYMS gene mRNA expression and EGFR gene mutation in stage Ⅲ A-N2 non-small-cell lung cancer tissue
10.3969/j.issn.1671-8348.2017.35.025
- VernacularTitle:ⅢA-N2期非小细胞肺癌组织TYMS基因mRNA表达与EGFR基因突变的相关性研究
- Author:
Zhe LI
1
;
Yang YANG
;
Yanfeng LIU
;
Ruibin XU
;
Tiehua RONG
;
Lanjun ZHANG
Author Information
1. 延安大学附属医院胸外科
- Keywords:
carcinoma,non-small-cell lung;
thymidylate synthetase;
receptor,epidermal growth factor;
gene expression;
gene mutation
- From:
Chongqing Medicine
2017;46(35):4977-4979
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between TYMS gene mRNA expression level and EGFR mutation in stage Ⅲ A-N2 non-small-cell lung cancer tissue (NSCLC).Methods The branched DNA-liquidchip technology (bDNA-LCT) and mutant-enriched liquidchip (MEL) technology were used to detect the TYMS mRNA expression and EGFR mutations at exon 19 and 21 in NSCLC tissues from 30 patients with stages Ⅲ A-N2 NSCLC,and the results were analyzed.Results Among 30 cases,low TYMS mRNA expression was in 14 cases (46.7 ％),middle to low TYMS mRNA expression in 7 cases (23.3 ％),middle mRNA expression in 7 cases (23.3 ％),middle to high TYMS mRNA expression in 0 case and high TYMS mRNA expression in 2 cases (6.7％);12 cases of EGFR mutation were detected out with the mutation rate of 40.0％,including 6 cases of exon 19 deletion and 6 cases of exon 21 missense mutation.The TYMS mRNA expression level was correlated with the EGFR mutation.The EGFR mutation commonly occurred in the tumor tissue of the patients with TYMS mRNA low expression (Z=-2.604,P=0.009).Conclusion The TYMS mRNA expression in NSCLC tissue is correlated with the EGFR gene mutation,which can provide references for the drug selection aiming at the patients with different conditions.
