Screening and identification of RNA interference sequence for human acute monocytic leukemia related antigen 22

VernacularTitle:人急性单核细胞白血病相关抗原22 RNA干扰序列的筛选及鉴定
Author: Liufang GU ¹ ; Pengyu ZHANG ; Xingmei CAO ; Wanggang ZHANG
Author Information

1. 710004,西安交通大学医学院第二附属医院血液内科
Keywords: Leukemia,monocytic,acute; RNA interference; Acute monocytic leukemia associated antigen 22
From: Journal of Leukemia & Lymphoma 2018;27(3):149-153
CountryChina
Language:Chinese
Abstract: Objective To screen the optimal RNA interference sequence of acute monocytic leukemia associated antigen 22 (MLAA-22) gene in order to study gene function of it. Methods MLAA-22 coding sequence (CDS) was cloned by reverse transcription polymerase chain reaction (RT-PCR) and the CDS was inserted in to pEGFP-N1-3FLAG vector to construct eukaryotic expression vector of MLAA-22. At the same time, four RNA interference sequences were designed and cloned to the vector. Expression vector and RNA interference vector were co-transfected into 293T cells, and the optimal RNA interference sequence was screened by fluorescence and Western blot analyses. Results MLAA-22 eukaryotic expression vector pEGFP-N1-3FLAG and four RNA interference vectors were successfully constructed. After co-transfected 293T cells, KD2 was selected as the optimal interference sequence of MLAA-22. Conclusion KD2 is an optimal interference sequence for targeting MLAA-22 antigen gene.