Dynamic visual analysis of neutrophils chemotaxis in peritoneal cavity of schizophrenic model in mice.
- Author:
Ning BAI
1
,
2
;
Ling Fei YANG
3
,
4
;
Li Hua AN
2
;
Wen WANG
2
;
Yun Qian LI
3
,
4
;
Hui SHENG
3
,
4
;
Tong WANG
3
,
4
;
Hua Kan LI
5
;
Lan YUAN
3
,
4
Author Information
1. Department of Pharmceutical,Jinzhou Medical University, Jinzhou 121000, Liaoning, China
2. Peking University Medical and Health Analysis Center,Beijing 100191,China.
3. Peking University Medical and Health Analysis Center,Beijing 100191,China
4. Department of Chemical Biology,Peking University School of Parmaceutical Sciences,Beijing 100191,China.
5. Department of Basic Science, Jinzhou Medical University, Jinzhou 121000, Liaoning, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Movement;
Chemokines;
Chemotaxis;
Disease Models, Animal;
Dizocilpine Maleate;
Mice;
Neutrophils/physiology*;
Peritoneal Cavity;
Rats;
Schizophrenia/physiopathology*
- From:
Journal of Peking University(Health Sciences)
2018;50(2):226-230
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare the differences of neutrophils chemotaxis ability in peritoneal cavity between normal rats and schizopherenic rats with cell dynamic visualization system.
METHODS:In the study,18 healthy Kunming rats were randomly divided into 3 groups which were control group (n=6), 0.3 mg/kg MK-801 treatment group (n=6), 0.6 mg/kg dizocilpine maleate (MK-801) treatment group(n=6), extracted neutrophils separately, and observed the morphology and counted under a microscope. Each group of cells was divided into two parts for chemotactic experiment, called chemokine agent treatment group and no chemokine agent treatment group respectively, indicating control 1, 0.3 mg/kg MK-801 treatment 1,0.6 mg/kg MK-801 treatment 1 and control 2, 0.3 mg/kg MK-801 treatment 2,0.6 mg/kg MK-801 treatment 2. The dynamic migration of cells was recorded using the NIS-Elements software, and TAXIScan Analyzer 2 software was used to select 30 cells (n=30) in each group of cells and analyze cells migration trajectory, speed and distance, and use pair test and One-Way analysis of variance for statistical analysis.
RESULTS:The number of neutrophils in control group, 0.3 mg/kg MK-801 treatment group and 0.6 mg/kg MK-801 treatment group were(1.00±0.03)×104/mL,(0.05±0.02)×104/mL,(0.32±0.01)×104/mL respectively, the differences of results were statistically significant(P<0.05).Under the effect of chemotactic agent,the directional migration capability of neutrophils in control group 1, 0.3 mg/kg MK-801 treatment group 1 and 0.6 mg/kg MK-801 treatment group 1 were(0.85±0.11) radian,(1.00±0.11) radian,(0.96±0.10) radian respectively (P<0.05); the migration velocities of neutrophils were (0.09±0.02) μm/s,(0.12±0.01) μm/s,(0.14±0.01) μm/s respectively (P<0.05);the migration distances of neutrophils were (94.26±0.02) μm,(134.61±0.01) μm,(156.19±0.01) μm respectively(P<0.05).
CONCLUSION:Compared with neutrophils in peritoneal cavity of control group, the neutrophils in peritoneal cavity of schizophrenic rats have stronger chemotactic movement ability.
