Effects of RGD peptides-grafted porous tantalum on morphological change of MG63 osteoblasts-tantalum conjunctive interface and expression of osteogenesis factors.
- Author:
Hong Quan GAN
1
;
Qian WANG
2
;
Hui ZHANG
3
;
Xin LIU
4
;
Hua Min DENG
1
;
Hui Ping SONG
1
;
Zhi Qiang WANG
1
;
Qi Jia LI
5
,
6
Author Information
1. Department of Orthopaedics, Affiliated Hospital, North China University of Science and Technology, Tangshan 063000, Hebei, China.
2. Department of Anatomy, Basic Medical College, North China University of Science and Technology, Tangshan 063000, Hebei, China.
3. Department of Joint Surgery, the Second Hospital of Tangshan, Tangshan 063000, Hebei, China.
4. Department of Clinical Laboratory, Kailuan General Hospital, Tangshan 063000, Hebei, China.
5. Experimental Center, North China University of Science and Technology
6. Tangshan 063000, Hebei, China.
- Publication Type:Journal Article
- MeSH:
Cell Adhesion;
Oligopeptides;
Osteoblasts/physiology*;
Osteogenesis;
Tantalum
- From:
Journal of Peking University(Health Sciences)
2018;50(1):176-182
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of the Arg-Gly-Asp polypeptedes (RGD) peptides-modified porous tantalum surface on osteoblasts morphology and expressions of osteogenesis factors, and to evaluate RGD peptides promotes junctura ossium of tantalum-bone interface in vivo.
METHODS:RGD peptides of different concentrations (1 g/L, 5 g/L, and 10 g/L) were loaded to porous tantalum slices with a diameter of 10 mm and a thickness of 3 mm by physical absorption. The 3rd generation of MG63 cells were co-cultured with tantalum and divided into 4 groups: Ta-cells (control) group, 1 g/L cells/Ta/RGD group, 5 g/L cells/Ta/RGD group, and 10 g/L cells/Ta/RGD group. Porous tantalum compo-sites and osteoblasts-tantalum interface were observed by scanning electron microscopy. The adhesion rate of osteoblasts was detected and immunocytochemistry was used to detect the expressions of filamentous actin (F-actin), osteocalcin (OC) and fibronectin (FN).
RESULTS:The scanning electron microscope (SEM) revealed that osteoblasts distributed on the surface of porous tantalum and secreted extracellular matrix on outside and inner of micro-pores. The osteoblasts adhesion rate on porous tantalum modified with RGD was higher than that in the unmodified porous tantalum at the end of 24, 48, and 72 hours. The best adhesion effect was got in 5 g/L cells/Ta/RGD group at hour 48 [(68.07±3.80) vs. (23.40±4.39), P<0.05]. The results of immunocytochemistry showed that the expressions intensity of F-actin, OC and FN in osteoblasts on porous tantalum modified groups with RGD were stronger than that in the unmodified groups, and the expressions of 5 g/L cells/Ta/RGD group were significantly higher than those in the 10 g/L group and 1 g/L group [OC: (18.08±0.08) vs. (15.14±0.19), P<0.05; (18.08±0.08) vs. (14.04±0.61), P<0.05. FN: (24.60±0.98) vs. (15.90±0.53), P<0.05; (24.60±0.98) vs. (15.30±0.42), P<0.05. F-actin: (29.20±1.31) vs. (24.50±1.51), P<0.05; (29.20±1.31) vs. (16.92±0.40), P<0.05]. Correspondingly F-actin in osteoblasts was showed in longitudinal arrangement, and the expressions intensity was stronger than those OC and FN.
CONCLUSION:The RGD peptides is beneficial to enhance adhesion of osteoblast, spreading and reorganization of cytoskeleton on porous tantalum surface and improve the interface morphology, further promoting osteoblasts-tantalum conjunctive interface osseointegration.
