Study on the anti-Candida krusei in vitro combined with tacrolimus and itraconazole
10.19405/j.cnki.issn1000-1492.2018.07.026
- VernacularTitle:他克莫司和伊曲康唑联合使用体外抗克柔念珠菌作用的研究
- Author:
Tingting GONG
1
;
Yunlei LIU
;
Zhongxin WANG
Author Information
1. 安徽医科大学第一附属医院检验科
- Keywords:
Candida krusei;
iraconazole;
tacrolimus
- From:
Acta Universitatis Medicinalis Anhui
2018;53(7):1114-1117
- CountryChina
- Language:Chinese
-
Abstract:
Objective The use of the antifungal agent itraconazole and the calcineurin inhibitor tacrolimus alone, together with the above two drugs, was evaluated in vitro for growth of Candida krusei. Whether or not the combina-tion is more effective than the antifungal alone can inhibit the growth of Candida krusei. Methods According to the guidelines of the American Institute of Clinical and Laboratory Standards, 12 Candida krusei strains were divided into blank control group, itraconazole group, tacrolimus group, and combination group by microdilution method, and then 12 the Candida krusei strain was incubated for 24 h and its growth was observed. Results In comparison with the alone use of isotriconazole in Candida krusei, the combined use of itraconazole,its minimal inhibitory con-centration was much less than that of alone, with a maximum multiple of 32 fold, a minimum multiple up to 4 times; the same, In comparison with the alone use of tacrolimus in Candida krusei, the combined use of tacroli-mus, its maximum multiple of 32 times, the lowest multiple of 2 times. Conclusion The combined use of itracon-azole and tacrolimus in 12 strains of Candida krusei displayed a powerful synergistic effect in vitro.
