Pravastatin sodium inhibits the release and activity of human peripheral blood neutrophil elastase induced by lipopolysaccharide
10.19405/j.cnki.issn1000-1492.2018.06.011
- VernacularTitle:普伐他汀钠对LPS诱导的中性粒细胞NE释放及活性的影响
- Author:
Yuhong CHEN
1
;
Min DAI
;
Xiaodong MEI
Author Information
1. 安徽医科大学附属安徽省立医院呼吸内科
- Keywords:
pravastatin sodium;
neutrophil elastase;
lipopolysaccharide;
myeloperoxidase
- From:
Acta Universitatis Medicinalis Anhui
2018;53(6):880-884
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of pravastatin sodium on the release and activity of neutrophil elastase ( NE) in human neutrophile granulocyte induced by lipopolysaccharide( LPS) . Methods Human neutrophile gran-ulocyte were isolated by Ficoll density gradient centrifugation, neutrophils and intracellular azurophilic granules were identified by myeloperoxidase( MPO) staining, LPS stimulated degranulation of neutrophils. The MPO activity in the supernatant was measured by colorimetric assay to determine the appropriate stimulus time and concentration, and neutrophil was treated with pravastatin sodium after LPS stimulation. ELISA mothed was used to detect the cell culture supernatant of NE content and NE activity was detected by colorimetric method. Results The content and activity of NE in the supernatant were significantly increased after LPS stimulation. The NE in the supernatant of the cells treated with pravastatin was significantly lower( P＜0.05) . Conclusion Pravastatin sodium can reduce neutrophil degranulation induced by LPS stimulation and decrease the release and activity of NE.
