Role of Met/HGF signal pathway on inhibiting autophagy in ischemia-reperfusion induced cardiomyocytes injury

VernacularTitle:Met/HGF通路抑制缺血再灌注心肌细胞自噬作用的研究
Author: Ao FENG ¹ ; Xianhe LIN
Author Information

1. 安徽医科大学第二临床医学院
Keywords: Met/HGF; autophagy; myocardial ischemia and reperfusion
From: Acta Universitatis Medicinalis Anhui 2017;52(12):1814-1818
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect and mechanism of autophagy inhibited by Met/HGF passway in alleviating myocardial ischemia and reperfusion injury.Methods Lsolated cardiomyocytes of rats were divided into 5 groups:control group,ischemia and reperfusion (IR) group,Met-transfection group,Met siRNA-transfection group,Met-transfection and HGF activator group.Over or less expressions of Met were controlled by the method of transfection.Cell proteins were extracted after IR injury.The expression of cell protein such as met,HGF,AMPK,PI3K,autophagy-related protein LC3B,Beclin-1 and apoptosis-related protein Bcl-2 were detected by Western blot.Results Compared with control group,Western blot results showed that the expression of AMPK and PI3K increased significantly by the method of over expression of Met and activation of HGF,the expression of autophagy-related protein LC3B and Beclin-1 decreased significantly at the same time.However,it showed an opposite trend in IR group and Met siRNA-transfection group.Conclusion Activation of the Met/HGF signal pathway inhibits autophagy and attenuates myocardial ischemia-reperfusion injury.