Therapeutic Role of Tangshenkang Granule () in Rat Model with Diabetic Nephropathy.

Shun-jin HU; Bing Shu; Hua Jin; Xiao-feng LI; Jia-rong Mao; Ke-jun Ren; Lei Gao; Li Yang; You-wan WU; Yong-jun Wang

Return

Therapeutic Role of Tangshenkang Granule () in Rat Model with Diabetic Nephropathy.

Author: Shun-Jin HU ¹ ; Bing SHU ² ; Hua JIN ³ ; Xiao-Feng LI ² ; Jia-Rong MAO ⁴ ; Ke-Jun REN ³ ; Lei GAO ⁵ ; Li YANG ⁵ ; You-Wan WU ⁵ ; Yong-Jun WANG ²
Author Information

1. Department of Internal Medicine, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, 230031, China. hushunjin@sohu.com.
2. Department of Orthopaedics, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.
3. Department of Internal Medicine, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, 230031, China.
4. Department of Pathology, Shanxi Hospital of Traditional Chinese Medicine, Xi'an, 710003, China.
5. Graduate School, Anhui University of Traditional Chinese Medicine, Hefei, 230038, China.
Publication Type:Journal Article
Keywords: Chinese medicine; Tangshenkang Granule; diabetic nephropathy; kidney function; kidney pathology; lipid metabolism
MeSH: Albuminuria; complications; Animals; Basement Membrane; drug effects; metabolism; Blood Urea Nitrogen; Body Weight; drug effects; Creatinine; blood; urine; Diabetic Nephropathies; blood; drug therapy; physiopathology; urine; Disease Models, Animal; Drugs, Chinese Herbal; pharmacology; therapeutic use; Hypertrophy; Kidney Function Tests; Kidney Glomerulus; drug effects; pathology; physiopathology; Lipid Metabolism; drug effects; Lipids; blood; Male; Rats, Sprague-Dawley
From: Chinese journal of integrative medicine 2018;24(8):600-605
CountryChina
Language:English
Abstract:
OBJECTIVETo evaluate the renal protective effect of Tangshenkang Granule () in a rat model of diabetic nephropathy (DN).
METHODSForty male Sprague-Dawley rats were randomly divided into control, DN, Tangshenkang and benazepril groups. DN model was established in the rats of DN, Tangshenkang and benazepril groups. Tangshenkang Granule solution and benazepril hydrochloride solution were intragastrically administered daily to the rats in the Tangshenkang and benazepril groups for 8 weeks, respectively. Urinary albumin and creatinine were detected. The albumin/creatinine (ACR) was calculated in addition to 24 h urinary protein (24-h UPr), serum creatinine (Scr), blood urea nitrogen (BUN), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), and creatinine clearance rate (Ccr). Right kidneys were harvested for pathological observation using periodic acid-silver methenamine-Masson staining. The average glomerular diameter (DG), average glomerular (AG) and mesangial areas (AM) were measured. The thickness of glomerular basement membrane (TGBM) was detected using transmission electron microscope.
RESULTSCompared with rats in the control group, rats in the DN group showed significantly decreased body weight, increased hypertrophy index, 24-h urinary volume, 24-h UPr, ACR, Scr, BUN, Ccr, blood lipids as well as renal pathological indices including DG, AG, AM, AM/AG and TGBM (P <0.05). Compared with the DN group, the weights of rats in the Tangshenkang and benazepril groups were significantly increased, and the renal hypertrophy indices were significantly decreased (P <0.05). The 24-h urinary volumes, ACR, 24-h UPr, Scr, BUN, Ccr, LDL, DG, AG, AM and TGBM were obviously decreased (P <0.05). Compared with the benazepril group, the Tangshenkang group showed significantly decreased levels of ACR, 24-h UPr, AG and AM (P <0.05).
CONCLUSIONSTangshenkang Granule decreased the urinary protein, attenuated the high glomerular filtration rate and improved lipid metabolism in DN rats, and prevented further injury induced by diabetic nephropathy.