Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice.

Gi Hee Jeong; William A Boisvert; Mei-zhu XI; Yi-lin Zhang; Young-bin Choi; Sunghun Cho; Sanghyun Lee; Changsun Choi; Bog-hieu Lee

Return

Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice.

Author: Gi Hee JEONG ¹ ; William A BOISVERT ² ; Mei-Zhu XI ¹ ; Yi-Lin ZHANG ¹ ; Young-Bin CHOI ¹ ; Sunghun CHO ³ ; Sanghyun LEE ³ ; Changsun CHOI ¹ ; Bog-Hieu LEE ⁴
Author Information

1. Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, 17546, Republic of Korea.
2. Center for Cardiovascular Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA.
3. Department of Integrative Plant Science, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, 17546, Republic of Korea.
4. Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, 17546, Republic of Korea. lbheelb@cau.ac.kr.
Publication Type:Journal Article
Keywords: Miscanthus sinensis var. purpurascens flower extract; hair growth; hepatocyte growth factor; human hair dermal papilla cell; transforming growth factor β1
MeSH: Animals; Antioxidants; pharmacology; Cell Count; Cell Proliferation; drug effects; Extracellular Signal-Regulated MAP Kinases; metabolism; Female; Flowers; chemistry; Hair Follicle; cytology; drug effects; growth & development; Hepatocyte Growth Factor; metabolism; Humans; Mast Cells; cytology; Mice, Inbred C57BL; Phosphorylation; drug effects; Plant Extracts; pharmacology; Poaceae; chemistry; RNA, Messenger; genetics; metabolism; Skin; metabolism; Stem Cell Factor; metabolism; Stress, Psychological; pathology; Substance P; metabolism; Transforming Growth Factor beta; genetics; metabolism; Vascular Endothelial Growth Factor A; genetics; metabolism; beta Catenin; metabolism
From: Chinese journal of integrative medicine 2018;24(8):591-599
CountryChina
Language:English
Abstract:
OBJECTIVESTo investigate the hair growth-promoting effect of Miscanthus sinensis var. purpurascens (MSP) flower extracton on in vitro and in vivo models.
METHODSMSP flower extract was extracted in 99.9% methanol and applied to examine the proliferation of human dermal papilla cells (hDPCs) in vitro at the dose of 3.92-62.50 μg/mL and hair growth of C57BL/6 mice in vivo at the dose of 1000 μg/mL. The expression of transforming growth factor β1 (TGF-β1), hepatocyte growth factor (HGF), β-catenin, substance P was measured by relative quantitative realtime polymerase chain reaction. Histopathological and immunohistochemical analysis were performed.
RESULTSMSP (7.81 μg/mL) down-regulated TGF-β1 and up-regulated HGF and β-catenin in hDPCs (P<0.01). MSP (1000 μg/mL)-treated mice showed the earlier transition of hair follicles from the telogen to the anagen phase. The number of mast cells was lower in the MSP-treated mice than in other groups (P<0.05 vs. NCS group). Substance P and TGF-β1 were expressed in hair follicles and skin of the MSP group lower than that in negative control. Stem cell factor in hair follicles was up-regulated in the MSP-treated mice (P<0.01).
CONCLUSIONSThe MSP flower extract may have hair growth-promotion activities.