Xuebijing Injection () and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury.

Shu-kun Zhang; Yu-zhen Zhuo; Cai-xia LI; Lei Yang; Hong-wei Gao; Xi-mo Wang

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Xuebijing Injection () and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury.

Author: Shu-Kun ZHANG ¹ ; Yu-Zhen ZHUO ¹ ; Cai-Xia LI ¹ ; Lei YANG ¹ ; Hong-Wei GAO ² ; Xi-Mo WANG ³
Author Information

1. Department of Cell and Molecular Biology, Institute of Acute Abdominal Diseases of Intergrated Traditional Chinese and Western Medicine, Tianjin Nankai Hospital, Tianjin, 300100, China.
2. Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
3. Department of Surgery, Tianjin Nankai Hospital, Tianjin, 300100, China. wangximo@126.com.
Publication Type:Journal Article
Keywords: Chinese medicine; Xuebijing Injection; lung injury; resolvin D1; sepsis
MeSH: Animals; CD18 Antigens; metabolism; Cell Adhesion; drug effects; Docosahexaenoic Acids; administration & dosage; pharmacology; therapeutic use; Drugs, Chinese Herbal; administration & dosage; pharmacology; therapeutic use; Injections; Intercellular Adhesion Molecule-1; metabolism; Leukocytes; drug effects; metabolism; pathology; Lung; drug effects; enzymology; pathology; Lung Injury; blood; complications; drug therapy; Male; Mice, Inbred C57BL; Peroxidase; metabolism; Sepsis; blood; complications; drug therapy; Survival Analysis
From: Chinese journal of integrative medicine 2018;24(4):272-277
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the effect of combined application of Xuebijing Injection ( , XBJ) and resolvin D1 (RvD1) on survival rate and the underlying mechanisms in mice with sepsisinduced lung injury.
METHODSThe cecal ligation and puncture (CLP) method was used to develop a mouse sepsis model. Specific pathogen free male C57BL/6 mice were randomly divided into 5 groups (n=20 each): sham, CLP, CLP+XBJ, CLP+RvD1 and CLP+XBJ+RvD1. After surgery, mice in the CLP+XBJ, CLP+RvD1 and CLP+XBJ+RvD1 groups were given XBJ (25 μL/g body weight), RvD1 (10 ng/g body weight), and their combination (the same dose of XBJ and RvD1), respectively. In each group, 12 mice were used to observe 1-week survival rate, while the rest were executed at 12 h. Whole blood was collected for flow cytometric analysis of leukocyte adhesion molecules CD18, lung tissues were harvested for observing pathological changes, and testing the activity of myeloperoxidase (MPO) and the expression of intercellular cell adhesion molecule 1 (ICAM-1).
RESULTSCompared with the CLP group, the histopathological damage of the lung tissues was mitigated, MPO activity was decreased in the CLP+XBJ and CLP+RvD1 groups (P<0.05). In addition, the 1-week survival rate was improved, proportion of CD18-expressing cells in whole blood and ICAM-1 protein expression in lung tissue were decreased in the CLP+XBJ+RvD1 group (P<0.05 or P<0.01).
CONCLUSIONSXBJ together with RvD1 could effectively inhibit leukocyte adhesion, reduce lung injury, and improve the survival rate of mice with sepsis.