Effects of Compound Zhebei Granule () Combined with Doxorubicin on Expression of Specific Surface Antigens in Mice with Transplanted KG-1a Cells.
- Author:
Yu ZHANG
1
;
Li HOU
2
;
Xin-Yi CHEN
3
Author Information
- Publication Type:Journal Article
- Keywords: Chinese medicine; acute myeloid leukemia; adriamycin; doxorubicin
- MeSH: Animals; Antigens, Surface; metabolism; Cell Line, Tumor; Cell Proliferation; drug effects; Cell Shape; drug effects; Doxorubicin; pharmacology; Drugs, Chinese Herbal; pharmacology; therapeutic use; Female; Humans; Mice, Inbred BALB C; Subcutaneous Tissue; drug effects; pathology; Xenograft Model Antitumor Assays
- From: Chinese journal of integrative medicine 2018;24(3):213-217
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of Compound Zhebei Granule (, CZG) combined with doxorubicin hydrochloride (adriamycin, ADM) on specific surface antigens in mice with KG-1a transplanted cells.
METHODSA subcutaneous tumor xenograft model was established by injection of the acute myeloid leukemia cell line KG-1a into the axillary flfl anks of BALB/c-nude mice. Twenty-four tumor bearing mice were divided into 4 groups according to a random number table, including normal saline control group, ADM group, high-dose CZG group, and mid-dose CZG group, with 6 mice in each group. Drug administration occurred on the 14th day after cell inoculation, and normal saline control group mice were gavaged with normal saline at 0.2 mL/10 g every other day. ADM group mice were intraperitoneally injected with ADM 1 mg/kg [conversion of adults, 40 mg/(m•d)] every other day. High- and mid-dose CZG groups mice were gavaged with CZG at the dose of 8 and 4 g/kg respectively every other day and intraperitoneally injected with ADM (1 mg/kg) every other day. The administration period lasted for 10 days. The tumor xenografts were made into mononuclear cell suspensions after dissection, and the expressions of specific surface antigens, including CD34CD38, CD34CD38CD123, CD34CD38CD96 and CD34CD38CD33, in KG-1a cell line tumor xenografts were detected by flfl ow cytometry.
RESULTSCompared with the control and ADM groups, expression of CD34CD38 in the two CZG groups was significantly lower (P<0.05). Compared with the control group, expression of CD34CD38CD123 in the two CZG groups decreased (P<0.05). The high-dose CZG group showed more obvious outcomes compared with the ADM group (P<0.05). Compared with the control and ADM groups, the expression of CD34CD38CD96 and CD34CD38CD33 in the two CZG groups decreased (P<0.05).
CONCLUSIONSCZG combined with doxorubicin could reduce the expression of CD34CD38, CD34CD38CD123, CD34CD38CD96 and CD34CD38CD33 in KG-1a cell line tumor xenografts, which shows that CZG could target leukemia stem cells and play a role in chemosensitization.
